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three color immunocytochemistry kit  (R&D Systems)
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Figure 6 ｜Efficacy assessments in the HIE rat model. (A) Animal experiment timeline. (B) Whole-brain and HE-staining images. The infarct area was significantly reduced in both the hNSC direct injection group and the hNSC-loaded SF hydrogel group compared with the HIE group. Scale bars: 1 cm. (C) Representative IHC images of GFAP in the cerebral cortex and hippocampal CA1, DG, and CA3 regions (scale bars: 100 μm). (D) Number of GFAP-positive cells. Data are presented as the mean ± SD (n = 10/group). *P < 0.05, **P < 0.01, ***P < 0.001 (one-way analysis of variance followed by the least significant difference post hoc test or Welch test with Dunnett post hoc test). CTX: Cerebral cortex; DG: dentate gyrus; ELISA: enzyme-linked immunosorbent assay; GFAP: glial fibrillary acidic protein; HE staining: hematoxylin–eosin staining; HIE: hypoxic-ischemic encephalopathy; hNSC: human-derived neural stem cell; IF: immunofluorescence; IHC: <t>immunohistochemistry;</t> ns: not significant; NSC: neural stem cell; SF: silk fibroin; STI: stereotaxic injection.
Three Color Immunocytochemistry Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/SC024/Human%2FMouse%2FRat+Neural+3-Color+Immunocytochemistry+Kit/10__4103_slash_nrr__nrr___d___24___01178-56-9-13
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three color immunocytochemistry kit - by Bioz Stars, 2026-10
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neural 3 colour immunocytochemistry kit  (R&D Systems)
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R&D Systems neural 3 colour immunocytochemistry kit
Figure 6 ｜Efficacy assessments in the HIE rat model. (A) Animal experiment timeline. (B) Whole-brain and HE-staining images. The infarct area was significantly reduced in both the hNSC direct injection group and the hNSC-loaded SF hydrogel group compared with the HIE group. Scale bars: 1 cm. (C) Representative IHC images of GFAP in the cerebral cortex and hippocampal CA1, DG, and CA3 regions (scale bars: 100 μm). (D) Number of GFAP-positive cells. Data are presented as the mean ± SD (n = 10/group). *P < 0.05, **P < 0.01, ***P < 0.001 (one-way analysis of variance followed by the least significant difference post hoc test or Welch test with Dunnett post hoc test). CTX: Cerebral cortex; DG: dentate gyrus; ELISA: enzyme-linked immunosorbent assay; GFAP: glial fibrillary acidic protein; HE staining: hematoxylin–eosin staining; HIE: hypoxic-ischemic encephalopathy; hNSC: human-derived neural stem cell; IF: immunofluorescence; IHC: <t>immunohistochemistry;</t> ns: not significant; NSC: neural stem cell; SF: silk fibroin; STI: stereotaxic injection.
Neural 3 Colour Immunocytochemistry Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
neural 3 colour immunocytochemistry kit - by Bioz Stars, 2026-10
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3 color immunocytochemistry kit  (R&D Systems)
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Figure 6 ｜Efficacy assessments in the HIE rat model. (A) Animal experiment timeline. (B) Whole-brain and HE-staining images. The infarct area was significantly reduced in both the hNSC direct injection group and the hNSC-loaded SF hydrogel group compared with the HIE group. Scale bars: 1 cm. (C) Representative IHC images of GFAP in the cerebral cortex and hippocampal CA1, DG, and CA3 regions (scale bars: 100 μm). (D) Number of GFAP-positive cells. Data are presented as the mean ± SD (n = 10/group). *P < 0.05, **P < 0.01, ***P < 0.001 (one-way analysis of variance followed by the least significant difference post hoc test or Welch test with Dunnett post hoc test). CTX: Cerebral cortex; DG: dentate gyrus; ELISA: enzyme-linked immunosorbent assay; GFAP: glial fibrillary acidic protein; HE staining: hematoxylin–eosin staining; HIE: hypoxic-ischemic encephalopathy; hNSC: human-derived neural stem cell; IF: immunofluorescence; IHC: <t>immunohistochemistry;</t> ns: not significant; NSC: neural stem cell; SF: silk fibroin; STI: stereotaxic injection.
3 Color Immunocytochemistry Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/SC024/Human%2FMouse%2FRat+Neural+3-Color+Immunocytochemistry+Kit/pm29701915-91-27-31
Average 94 stars, based on 1 article reviews
3 color immunocytochemistry kit - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
neural 3 color immunocytochemistry kit  (R&D Systems)
94
R&D Systems neural 3 color immunocytochemistry kit
Figure 6 ｜Efficacy assessments in the HIE rat model. (A) Animal experiment timeline. (B) Whole-brain and HE-staining images. The infarct area was significantly reduced in both the hNSC direct injection group and the hNSC-loaded SF hydrogel group compared with the HIE group. Scale bars: 1 cm. (C) Representative IHC images of GFAP in the cerebral cortex and hippocampal CA1, DG, and CA3 regions (scale bars: 100 μm). (D) Number of GFAP-positive cells. Data are presented as the mean ± SD (n = 10/group). *P < 0.05, **P < 0.01, ***P < 0.001 (one-way analysis of variance followed by the least significant difference post hoc test or Welch test with Dunnett post hoc test). CTX: Cerebral cortex; DG: dentate gyrus; ELISA: enzyme-linked immunosorbent assay; GFAP: glial fibrillary acidic protein; HE staining: hematoxylin–eosin staining; HIE: hypoxic-ischemic encephalopathy; hNSC: human-derived neural stem cell; IF: immunofluorescence; IHC: <t>immunohistochemistry;</t> ns: not significant; NSC: neural stem cell; SF: silk fibroin; STI: stereotaxic injection.
Neural 3 Color Immunocytochemistry Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/SC024/Human%2FMouse%2FRat+Neural+3-Color+Immunocytochemistry+Kit/pmc03962683-51-7-12
Average 94 stars, based on 1 article reviews
neural 3 color immunocytochemistry kit - by Bioz Stars, 2026-10
94/100 stars
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Figure 6 ｜Efficacy assessments in the HIE rat model. (A) Animal experiment timeline. (B) Whole-brain and HE-staining images. The infarct area was significantly reduced in both the hNSC direct injection group and the hNSC-loaded SF hydrogel group compared with the HIE group. Scale bars: 1 cm. (C) Representative IHC images of GFAP in the cerebral cortex and hippocampal CA1, DG, and CA3 regions (scale bars: 100 μm). (D) Number of GFAP-positive cells. Data are presented as the mean ± SD (n = 10/group). *P < 0.05, **P < 0.01, ***P < 0.001 (one-way analysis of variance followed by the least significant difference post hoc test or Welch test with Dunnett post hoc test). CTX: Cerebral cortex; DG: dentate gyrus; ELISA: enzyme-linked immunosorbent assay; GFAP: glial fibrillary acidic protein; HE staining: hematoxylin–eosin staining; HIE: hypoxic-ischemic encephalopathy; hNSC: human-derived neural stem cell; IF: immunofluorescence; IHC: immunohistochemistry; ns: not significant; NSC: neural stem cell; SF: silk fibroin; STI: stereotaxic injection.

Journal: Neural Regeneration Research

Article Title: An innovative treatment for hypoxic-ischemic encephalopathy: Silk fibroin nanomaterials improve neural stem cell axon formation and facilitate cognitive improvement

doi: 10.4103/nrr.nrr-d-24-01178

Figure Lengend Snippet: Figure 6 ｜Efficacy assessments in the HIE rat model. (A) Animal experiment timeline. (B) Whole-brain and HE-staining images. The infarct area was significantly reduced in both the hNSC direct injection group and the hNSC-loaded SF hydrogel group compared with the HIE group. Scale bars: 1 cm. (C) Representative IHC images of GFAP in the cerebral cortex and hippocampal CA1, DG, and CA3 regions (scale bars: 100 μm). (D) Number of GFAP-positive cells. Data are presented as the mean ± SD (n = 10/group). *P < 0.05, **P < 0.01, ***P < 0.001 (one-way analysis of variance followed by the least significant difference post hoc test or Welch test with Dunnett post hoc test). CTX: Cerebral cortex; DG: dentate gyrus; ELISA: enzyme-linked immunosorbent assay; GFAP: glial fibrillary acidic protein; HE staining: hematoxylin–eosin staining; HIE: hypoxic-ischemic encephalopathy; hNSC: human-derived neural stem cell; IF: immunofluorescence; IHC: immunohistochemistry; ns: not significant; NSC: neural stem cell; SF: silk fibroin; STI: stereotaxic injection.

Article Snippet: NSC differentiation efficiency was evaluated using a human/mouse/rat neural three-color immunocytochemistry kit (SC024, R&D Systems) according to the manufacturer’s instructions.

Techniques: Staining, Injection, Enzyme-linked Immunosorbent Assay, Derivative Assay, Immunofluorescence, Immunohistochemistry